Pharmacokinetics and pharmacodynamics of furosemide after oral administration to horses     

Johansson AM  Gardner SY  Levine JF  Papich MG  Lafevers DH  Goldman RB  Sheets MK  Atkins CE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(5):739-743

Furosemide is the most common diuretic drug used in horses. Furosemide is routinely administered as IV or IM bolus doses 3-4 times a day. Administration PO is often suggested as an alternative, even though documentation of absorption and efficacy in horses is lacking. This study was carried out in a randomized, crossover design and compared 8-hour urine volume among control horses that received placebo, horses that received furosemide at 1 mg/kg PO, and horses that received furosemide at 1 mg/kg IV. Blood samples for analysis of plasma furosemide concentrations, PCV, and total solids were obtained at specific time points from treated horses. Furosemide concentrations were determined by reversed-phase high-performance liquid chromatography with fluorescent detection. Systemic availability of furosemide PO was poor, erratic, and variable among horses. Median systemic bioavailability was 5.4% (25th percentile, 75th percentile: 3.5, 9.6). Horses that received furosemide IV produced 7.4 L (7.1, 7.7) of urine over the 8-hour period. The maximum plasma concentration of 0.03 microg/mL after administration PO was not sufficient to increase urine volume compared with control horses (1.2 L [1.0, 1.4] PO versus 1.2 L [1.0, 1.4] control). There was a mild decrease in urine specific gravity within 1-2 hours after administration of furosemide PO, and urine specific gravity was significantly lower in horses treated with furosemide PO compared with control horses at the 2-hour time point. Systemic availability of furosemide PO was poor and variable. Furosemide at 1 mg/kg PO did not induce diuresis in horses.  相似文献   

Atrial fibrillation in cats: 50 cases (1979-2002)     

Côté E  Harpster NK  Laste NJ  MacDonald KA  Kittleson MD  Bond BR  Barrett KA  Ettinger SJ  Atkins CE 《Journal of the American Veterinary Medical Association》2004,225(2):256-260

OBJECTIVE: To determine signalment, clinical signs, diagnostic findings, treatment, and outcome for cats with atrial fibrillation (AF). DESIGN: Retrospective study. ANIMALS: 50 cats. PROCEDURE: Medical records of cats that met criteria for a diagnosis of AF (ECG consisting of at least 2 leads, clear absence of P waves, supraventricular rhythm, and convincingly irregularly irregular rhythm) and had undergone echocardiography were reviewed. RESULTS: There were 41 males (37 castrated) and 9 females (7 spayed). Forty-one were of mixed breeding; 9 were purebred. Mean +/- SD age was 10.2 +/- 3.7 years. The most common chief complaints were dyspnea, aortic thromboembolism, and lethargy. In 11 cats, AF was an incidental finding. Mean +/- SD ventricular rate was 223 +/- 36 beats/min. The most common echocardiographic abnormalities were restrictive or unclassified cardiomyopathy (n = 19), concentric left ventricular hypertrophy (18), and dilated cardiomyopathy (6). Mean +/- SD left atrial-to-aortic diameter ratio (n = 39) was 2.55 +/- 0.80. The most common thoracic radiographic findings were cardiomegaly, pleural effusion, and pulmonary edema. Median survival time (n = 24) was 165 days (range, 0 to 1,095 days). Eight of 24 cats lived for > or = 1 year after a diagnosis of AF was made. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that AF occurs primarily in older adult male cats with structural heart disease severe enough to lead to atrial enlargement. Atrial fibrillation in these cats was most commonly first detected when signs of decompensated cardiac disease were evident, but also was commonly identified as an incidental finding.  相似文献   

Genetic parameters for carcass and meat quality traits and their relationships to liveweight and wool production in hogget Merino rams.     

J C Greeff  E Safari  N M Fogarty  D L Hopkins  F D Brien  K D Atkins  S I Mortimer  J H J van der Werf 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2008,125(3):205-215

Genetic parameters for carcass and meat quality traits of about 18-month-old Merino rams (n = 5870), the progeny of 543 sires from three research resource flocks, were estimated. The estimates of heritability for hot carcass weight (HCW) and the various fat and muscle dimension measurements were moderate and ranged from 0.20 to 0.37. The brightness of meat (colour L*, 0.18 +/- 0.03 standard error) and meat pH (0.22 +/- 0.03) also had moderate estimates of heritability, although meat relative redness (colour a*, 0.10 +/- 0.03) and relative yellowness (colour b*, 0.10 +/- 0.03) were lower. Heritability estimates for live weights were moderate and ranged from 0.29 to 0.41 with significant permanent maternal environmental effects (0.13 to 0.10). The heritability estimates for the hogget wool traits were moderate to high and ranged from 0.27 to 0.60. The ultrasound measurements of fat depth (FATUS) and eye muscle depth (EMDUS) on live animals were highly genetically correlated with the corresponding carcass measurements (0.69 +/- 0.09 FATC and 0.77 +/- 0.07 EMD). Carcass tissue depth (FATGR) had moderate to low genetic correlations with carcass muscle measurements [0.18 +/- 0.10 EMD and 0.05 +/- 0.10 eye muscle area (EMA)], while those with FATC were negative. The genetic correlation between EMD and eye muscle width (EMW) was 0.41 +/- 0.08, while EMA was highly correlated with EMD (0.89 +/- 0.0) and EMW (0.78 +/- 0.04). The genetic correlations for muscle colour with muscle measurements were moderately negative, while those with fat measurements were close to zero. Meat pH was positively correlated with muscle measurements (0.14 to 0.17) and negatively correlated with fat measurements (-0.06 to -0.18). EMDUS also showed a similar pattern of correlations to EMD with meat quality indicator traits, although FATUS had positive correlations with these traits which were generally smaller than their standard error. The genetic correlations among the meat colour traits were high and positive while those with meat pH were high and negative, which were all in the favourable direction. Generally, phenotypic correlations were similar or slightly lower than the corresponding genetic correlations. There were generally small to moderate negative genetic correlations between clean fleece weight (CFW) and carcass fat traits while those with muscle traits were close to zero. As the Merino is already a relatively lean breed, this implies that particular attention should be given to this relationship in Merino breeding programmes to prevent the reduction of fat reserves as a correlated response to selection for increased fleece weight. The ultrasound scan traits generally showed a similar pattern to the corresponding carcass fat and muscle traits. There was a small unfavourable genetic correlation between CFW and meat pH (0.19 +/- 0.07).  相似文献   

ACVIM consensus guidelines for the diagnosis and treatment of myxomatous mitral valve disease in dogs     

Bruce W. Keene  Clarke E. Atkins  John D. Bonagura  Philip R. Fox  Jens Hggstrm  Virginia Luis Fuentes  Mark A. Oyama  John E. Rush  Rebecca Stepien  Masami Uechi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(3):1127-1140

This report, issued by the ACVIM Specialty of Cardiology consensus panel, revises guidelines for the diagnosis and treatment of myxomatous mitral valve disease (MMVD, also known as endocardiosis and degenerative or chronic valvular heart disease) in dogs, originally published in 2009. Updates were made to diagnostic, as well as medical, surgical, and dietary treatment recommendations. The strength of these recommendations was based on both the quantity and quality of available evidence supporting diagnostic and therapeutic decisions. Management of MMVD before the onset of clinical signs of heart failure has changed substantially compared with the 2009 guidelines, and new strategies to diagnose and treat advanced heart failure and pulmonary hypertension are reviewed.  相似文献   

Detection and quantification of airborne inoculum of Sclerotinia sclerotiorum using quantitative PCR     

S. L. Rogers  S. D. Atkins  J. S. West 《Plant pathology》2009,58(2):324-331

This paper reports the development of a new specific diagnostic technique to accurately quantify airborne inoculum of Sclerotinia sclerotiorum and discusses its potential use in disease-forecasting schemes, using examples of three contrasting epidemic seasons: 2007, when there was a severe epidemic of sclerotinia stem rot (SSR) in England and high numbers of airborne ascospores were trapped at Rothamsted, and, in contrast, 2003 and 2004, when the incidence of SSR in England was low and low numbers of airborne ascospores were trapped at Rothamsted. DNA was extracted from wax-coated plastic tapes, such as those used in Burkard (Hirst-type) spore traps and rotating-arm traps. A SYBR-green quantitative PCR (qPCR) method produced a linear relationship between ascospore numbers and S. sclerotiorum DNA (mean 0·35 pg DNA per spore) and was able to detect DNA representing as few as two ascospores. The technique was insensitive to DNA of the host plant, Brassica napus , and other plant pathogens, including Sclerotinia minor , S. trifoliorum and Botrytis cinerea , and common airborne fungal genera such as Cladosporium and Penicillium . There was no relationship between rainfall and numbers of airborne ascospores of S. sclerotiorum present at Rothamsted during the period of infection in the severe SSR season (2007).  相似文献   

Evidence of differences in seed dormancy among populations of Bromus sterilis     

Peters  Atkins  Brain 《Weed Research》2000,40(5):467-478

Seeds of 40 populations of Bromus sterilis L. were collected in the southern and midland counties of England over a 2‐year period and grown outdoors in pots in two subsequent years. In the first year, seeds were tested in a 12‐h dark/12‐h light regime at 15 °C and in the second year the seeds were tested both in the dark/light regime and in the dark at 15 °C. There was a wide range in the degree of enforced dormancy given by the dark/light regime. Germination of freshly collected seeds in the dark/light regime after 21 days ranged from 44% to 97% in the populations tested in the first year and from 19% to 97% in populations tested in the second year. Induced dormancy was caused by light in two populations. Seeds had little innate dormancy apart from in two populations which gave 64% and 68% germination, respectively, in the dark after 21 days. A field trial in which seeds of a selected range of six populations were sown on the soil surface after harvest (August 8), showed that populations predicted to be inhibited by light in laboratory tests were also inhibited by light in the field and, depending upon the population, there was between 4% and 54% of the seeds remaining ungerminated by October 23 in the year of planting. By June of the following year, 36% of the seeds sown on the soil surface in one population still remained viable and ungerminated. The agricultural significance of the results is discussed.  相似文献   

An overlapping protein-coding region in influenza A virus segment 3 modulates the host response   总被引：1，自引：0，他引：1  

Jagger BW  Wise HM  Kash JC  Walters KA  Wills NM  Xiao YL  Dunfee RL  Schwartzman LM  Ozinsky A  Bell GL  Dalton RM  Lo A  Efstathiou S  Atkins JF  Firth AE  Taubenberger JK  Digard P 《Science (New York, N.Y.)》2012,337(6091):199-204

Influenza A virus (IAV) infection leads to variable and imperfectly understood pathogenicity. We report that segment 3 of the virus contains a second open reading frame ("X-ORF"), accessed via ribosomal frameshifting. The frameshift product, termed PA-X, comprises the endonuclease domain of the viral PA protein with a C-terminal domain encoded by the X-ORF and functions to repress cellular gene expression. PA-X also modulates IAV virulence in a mouse infection model, acting to decrease pathogenicity. Loss of PA-X expression leads to changes in the kinetics of the global host response, which notably includes increases in inflammatory, apoptotic, and T lymphocyte-signaling pathways. Thus, we have identified a previously unknown IAV protein that modulates the host response to infection, a finding with important implications for understanding IAV pathogenesis.  相似文献   

Genetic basis of control of <Emphasis Type="Italic">Rhynchosporium secalis</Emphasis> infection and symptom expression in barley     

M. E. Looseley  A. C. Newton  S. D. Atkins  B. D. L. Fitt  B. A. Fraaije  W. T. B. Thomas  R. Keith  M. Macaulay  J. Lynott  D. Harrap 《Euphytica》2012,184(1):47-56

The genetic basis of several different components of resistance to Rhynchosporium secalis in barley was investigated in a mapping population derived from a cross between winter and spring barley types. Both the severity of visual disease symptoms and amount of R. secalis DNA in leaf tissues were assessed in field trials in Scotland in the 2007/2008 and 2008/2009 growing seasons. Relative expression of symptoms was defined as the residual values from a linear regression of amount of R. secalis DNA against visual plot disease score at GS 50. Amount of R. secalis DNA and visual disease score were highly correlated traits and identified nearly identical QTL. The genetic control of relative expression of symptoms was less clear. However, a QTL on chromosome 7H was identified as having a significant effect on the expression of visual disease symptoms relative to overall amount of R. secalis colonisation.  相似文献   

Cutting balloon catheterization for interventional treatment of cor triatriatum dexter: 2 Cases     

Nicole LeBlanc  Teresa C. DeFrancesco  Allison K. Adams  Clark E. Atkins  Sandra P. Tou  James Curt Fudge  Bruce W. Keene 《Journal of Veterinary Cardiology》2012,14(4):525-530

Cutting balloon dilatation was performed successfully in two dogs with cor triatriatum dexter and clinical signs of ascites. The cutting balloon catheter uses incisional microtomes embedded in a balloon catheter. During balloon expansion, these microtomes incise the adjacent tissue, decreasing circumferential wall stress. This theoretically reduces both the likelihood of fracturing the adjacent tissues in an uncontrolled manner and the potential neoproliferative response to standard balloon dilatation and the subsequent incidence of re-stenosis. In both cases described, clinical signs resolved completely following cutting balloon dilatation of the anomalous membrane. Based on the outcome of these 2 cases, cutting balloon dilatation appears to be a viable treatment option for dogs affected with cor triatriatum dexter.  相似文献   

Essential oil spray reduces clinical signs of insect bite hypersensitivity in horses     

A Cox  K Wood  G Coleman  AJ Stewart  F-R Bertin  H Owen  WW Suen  CE Medina-Torres 《Australian veterinary journal》2020,98(8):411-416

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